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Anti-murine CD31 (PECAM-1) for formalin-fixed paraffin (FFPE) sections
The antibody clone SZ31 is the first clone producing an antibody that specifically reacts with murine CD31 in standard formalin-fixed paraffin-embedded tissues.

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Anti-murine CD31 (PECAM-1) for formalin-fixed paraffin (FFPE) sections

Dear Ladies and Gentlemen

The antibody clone SZ31 is the first clone producing an antibody that specifically reacts with murine CD31 in standard
formalin-fixed paraffin-embedded tissues.

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Immunostaining of endothelial cells in vessels and capillaries of murine lung and liver with anti-CD31 antibody clone SZ31.

The antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of murine adenocarcinoma.

Specific staining of endothelial cells in vessels and capillaries of murine spinal cord and skeletal muscle with antibody clone SZ31.

All pictures courtesy of Prof. Dr. H. Stein, Institute of Pathology, Charité, Berlin, Germany.


The antibody clone SZ31 is the first clone producing an antibody that specifically reacts with murine CD31 in standard
formalin-fixed paraffin-embedded tissues. CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1)
is a 130kDa integral membrane glycoprotein and a member of the immunoglobulin superfamily.
This haptotaxin is expressed on the surface of embryonic and adult endothelial cells, monocytes, thrombocytes,
granulocytes and certain subgroups of T-cells. CD31 mediates cell-cell interactions, e.g., in an inflammatory process
during which the number of this receptor is greatly enhanced by guiding a granulozyte along a gradient of increasing densitiy
of CD31 molecules to the end of an endothelial cell where diapedesis is possible. CD31/PECAM-1 mediated endothelial
cell-cell interactions play a major role in angiogenesis. Until now pathophysiological studies of CD31/PECAM-1 in murine
model systems had limitations because standard formalin-fixed sections were problematic.
Monoclonal antibodies from the clone SZ31 eliminate these restrictions by allowing high quality immunohistochemical
analysis of standard formalin-fixed paraffin sections in mice.

References
1. Hölscher M et al. Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out
protects from acute myocardial ischemic injury. J.Biol.Chem. 286:11185-11194, 2011.
2. Kröger C et al. Placental vasculogenesis is regulated by keratin-mediated hyperoxia
in murine decidual tissues. Am.J.Pathol. 178(4): 1587-1590, 2011.

Catalog Number: DIA 310
Size: 100 ug
Specificity: Murine CD31 = PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) on endothelial cells
Class: Monoclonal
Clone: SZ31
Host: Rat
Isotype: IgG2a
Form: Lyophilized powder
Applications: Immunohistochemistry, either formalin-fixed paraffin (FFPE) or frozen; Immunoblotting

Please do not hesitate to contact us for further information.

Thank you for your attention and best regards

Andy Uhlig
Marketing and Sales Manager Distribution

 
MILAN ANALYTICA AG
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fon +41 (0)61 845 99 88
fax +41 (0)61 845 99 87
e-mail: andy.uhlig@milananalytica.ch
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