Milan Analytica AG

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Ladies and Gentlemen,

Recently Jackson Immunoresearch introduced Alexa Fluor® 680 and Alexa Fluor® 790 for high-sensitivity Western blots. These far-red and infrared dyes are extremely bright.

Furthermore Cy2 and Cy5 dyes conjugated to selected antibodies and streptavidin were reintroduced. These conjugates where brought back because Cy2, Cy3, and Cy5 dyes much better withstand the harsh dehydration conditions required for transfer to hydrophobic mounting media. In plastic mounting media Cy2, Cy3, and Cy5 are brighter than other dyes and even brighter than they are in aqueous media.

MILAN Analytica AG is the official distributor for Jackson ImmunoResearch products in Switzerland



Figure 2. Double immunofluorescence staining on a Western blot using Alexa Fluor® 680 far-red dye and Alexa Fluor® 790 infrared dye. Mouse IgG and goat IgG were reduced and denatured with β-mercaptoethanol and SDS. The heavy and light chains were separated by electrophoresis in SDS-PAGE, transferred to nitrocellulose, and double labeled with a 1:100,000 dilution of Alexa Fluor® 790-goat anti-mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot, 115-655-205)(green) to detect heavy chains and a 1:100,000 dilution of Alex Fluor® 680-goat anti-mouse IgG, light chain specific (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig, 115-625-174)(red) to detect light chains. Fluorescence was imaged in a LiCor Odyssey imager. Goat IgG was used as a background control. Note the faint bands of goat IgG heavy and light chains attesting to the extreme brightness of the dyes even at a dilution of 1:100,000. Blot

Please do not hesitate to contact us for further information.
Thank you for your consideration and best regards

Marc Vegh
Front Office and Laboratory Manager


Baslerstrasse 15
4310 Rheinfelden/Basel

fon +41 (0)61 845 99 88
fax +41 (0)61 845 99 87

MILAN ANALYTICA AG • 4310 Rheinfelden • Switzerland • phone 0041 61 845 99 88
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